PERFORMING THE SIBO TEST – SEATTLE, WA
PERFORMING THE BREATH TEST
IMPORTANT: Please watch our step-by-step instructional video at least 48 hours before you plan to begin your SIBO test. The video can be found on our home page -> WATCH INSTRUCTIONAL VIDEO.
The collection of ten breath samples will be performed as follows:
Before you begin, complete all labels with patient’s name and date of collection.
Mix the lactulose or glucose in ~8 ounces of water and set it aside. Do not drink it until after you have collected your first sample (your baseline sample).
Hold the EasySampler breath collector in one hand and a collection tube in the other.
Breathe normally. Do not take a deep breath as this may invalidate your sample. Place the breath collector to your mouth and begin a 4- second exhalation. As you begin to exhale, the bag will inflate, capturing the gases from your lungs inside. Note that the bag has a small hole near the mouthpiece, which is intentional and allows air from your lungs to continuously pass into the bag.
After the initial 2 seconds, and while continuing to exhale, push the collection tube firmly into the breath collector to pierce the septum of the collection tube, and hold it in place for the final two seconds, which completes the 4-second exhalation.
Before you begin, complete all labels with patient’s name and date of collection.
Mix the lactulose or glucose in ~8 ounces of water and set it aside. Do not drink it until after you have collected your first sample (your baseline sample).
Hold the EasySampler breath collector in one hand and a collection tube in the other.
Breathe normally. Do not take a deep breath as this may invalidate your sample. Place the breath collector to your mouth and begin a 4- second exhalation. As you begin to exhale, the bag will inflate, capturing the gases from your lungs inside. Note that the bag has a small hole near the mouthpiece, which is intentional and allows air from your lungs to continuously pass into the bag.
After the initial 2 seconds, and while continuing to exhale, push the collection tube firmly into the breath collector to pierce the septum of the collection tube, and hold it in place for the final two seconds, which completes the 4-second exhalation.
BREATH SAMPLE |
COLLECTION TIME |
|---|---|
| 1 (Baseline) | Just before drinking |
| 2 | 20 min after drinking |
| 3 | 40 min after drinking |
| 4 | 60 min after drinking |
| 5 | 80 min after drinking |
| 6 | 100 min after drinking |
| 7 | 120 min after drinking |
| 8 | 140 min after drinking |
| 9 | 160 min after drinking |
| 10 | 180 min after drinking |
Remove the collection tube, and record the collection time on the collection tube label. When finished, place the collection tube inside one of the plastic bubble wrap bags. You have now completed your baseline sample.
Drink the entire lactulose or glucose solution at once, and set a timer for 20 minutes.
Repeat the breath collection procedure outlined above for each breath sample until all 10 samples have been collected.
BREATH SAMPLE |
COLLECTION TIME |
|---|---|
| 1 (Baseline) | Just before drinking |
| 2 | 20 min after drinking |
| 3 | 40 min after drinking |
| 4 | 60 min after drinking |
| 5 | 80 min after drinking |
| 6 | 100 min after drinking |
| 7 | 120 min after drinking |
| 8 | 140 min after drinking |
| 9 | 160 min after drinking |
| 10 | 180 min after drinking |
IMPORTANT CONSIDERATIONS
- For tests containing lactulose: Lactulose is made from lactose and galactose and a small amount of lactose and galactose is present in the lactulose solution. This test is not recommended for those who are allergic to lactulose or lactose or are on lactose- or galactose- restricted diets.
- Ensure that your breath is collected precisely at 20 minute intervals as the accuracy of the test depends on this.
- DO NOT attempt to disassemble the breath collector or remove the rubber sheath from the needle inside.
- DO NOT eat or drink anything during the test or in the morning on the day of the test.
- Each breath collection involves a single, 4-second exhalation where the collection tube is inserted after the initial 2 seconds. DO NOT insert the collection tube too soon as this may invalidate your sample.